Most recent answer. Reza Ghahremani. University of Tehran. Agar is definitely the best and most widely used culture medium. Phytagel is a very solidity medium and is only suitable for detecting MacConkey agar has colonies that appear either red or white based on their _____. antibiotic susceptibility ability to lyse red blood cells reaction to the Gram stain. ability to ferment lactose The temperature controlled chamber, operated in the range of 20oC to 45oC to encourage the multiplication of bacteria at the second step of the 5 I's Chocolate agar (CA) is an enriched medium for the isolation and identification of fastidious bacteria. Defibrinated blood is used to manufacture CA, but this expensive product is not always affordable for companies in developing countries. Blood powder (BP) is potentially a cheaper alternative, although its pre-treatment using autoclaving can Likewise, it becomes nutrient agar when 2-3% agar is added. For purposes of diagnosis, this is the simplest and most common media used in laboratories. A semisolid medium that permits the propagation of motile bacteria can be produced if the concentration of agar is decreased. Examples – peptone water, nutrient agar and glucose broth. Agar Agar is a plant-based gelatin-like substance that is derived from red seaweed. It’s roughly 80 per cent fibre, and acts as a thickening agent when added to water or other liquids. When added to water, it thickens and in the right amounts can turn into jelly - creating a whole world of desert opportunities. The difference in isolation of polymicrobial growth between HiCrome TM UTI agar and either CLED agar or MAC agar was found to be statistically significant (P < 0.05). Table 3. Rate of isolation of uropathogens in MAC agar, CLED Agar, HiCrome UTI Agar Isolation on Blood agar . Pneumococci frequently require enriched media and increased CO 2 tension for initial isolation. They are usually isolated on Blood agar and incubated in a candle jar (a closed container in which a lit candle is placed to remove O 2 and increase CO 2) at 37C. On Blood agar, colonies appear small, shiny, and translucent. iOYtS.

difference between agar and agar agar